DB43/T2673—2023 43 湖南省地方标准 ICS CCS 67.060 B 22 发 布 湖南省市场监督管理局2023-0 8发布-22 2023-11实施-2 2番茄花粉保藏技术规程 Code of practice for preservation of tomato pollen DB43/T 2673—2023 I 目 次 前言 ································ ································ ································ ························ Ⅲ 1 范围 ································ ································ ································ ····················· 1 2 规范性引用文件 ································ ································ ································ ······ 1 3 术语和定义 ································ ································ ································ ············ 1 4 花朵采摘 ································ ································ ································ ··············· 1 5 花粉采集 ································ ································ ································ ··············· 1 6 花粉保藏 ································ ································ ································ ··············· 1 7 花粉解冻 ································ ································ ································ ··············· 2 8 花粉回收 ································ ································ ································ ··············· 2 附录A（资料性） 番茄花朵的图样式 ································ ································ ············· 3 DB43/T 2673—2023 II DB43/T 2673—2023 III 前 言 本文件按照 GB/T 1.1 —2020《标准化工作导则 第1部分：标准化文件的结构和起草规则》的规 定起草。 请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。 本文件由湖南省农业农村厅提出。 本文件由湖南省农 业标准化技术委员会归口。 本文件起草单位：湖南省蔬菜研究所、湖南省植物保护研究所、湖南邦博农业科技有限公司、长沙 市望城区绿地生态农庄有限公司、宁乡辣美椒甜农业科技有限公司。 本文件主要起草人：白占兵、张战泓、欧阳娴、李雪峰、刘勇、张松柏、张鑫、张宇、刘龙飞、徐 灿、何文平、肖胜蓝。 DB43/T 2673—2023 IV DB43/T 2673—2023 1 番茄花粉保藏技术规程 1 范围 本文件规定了番茄花粉保藏技术的花朵采摘、花粉采集、花粉保藏、花粉解冻、花粉回收等内容。 本文件适用于番茄杂交种子生产过程中的花粉保藏。 2 规范性引用文件 下列文件中的内 容通过文中的规范性引用而构成本文件必不可少的条款。 其中， 注日期的引用文件， 仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本 文件。 本文件没有规范性引用文件。 3 术语和定义 下列术语和定义适用于本文件。 3.1 番茄花粉 tomato pollen 番茄植株雄蕊中花药产生的粉状物质。 4 花朵采摘 宜选择晴天上午，露水散去后，采摘父本植株上花瓣开放、花药未裂开的花朵，参见附录 A的图样式。 5 花粉采集 将采摘的新鲜花朵摊开，抢在花药未裂开前，用镊子取出花药置于蜡光纸上，置于温度 20 ℃～ 28 ℃的环境干燥 6 h～8 h，让花药开裂。将裂开的花药置于 60 目～80 目的筛网上过筛去杂，收集的 花粉放置于干燥器中干燥至不结块，分装花粉于冷冻管中密封。 6 花粉保藏 6.1 低温保藏 花粉冷冻管在 4 ℃环境中可保藏 5 d～7 d；-20 ℃环境中可保藏约 30 d。 6.2 超低温保藏 花粉冷冻管直接置于液氮（ -196 ℃）中或者置于液氮（ -196 ℃）中3 min～5 min骤冷后在 -80 ℃ DB43/T 2673—2023 2 条件下均可保藏 1年左右。注意及时补充液氮。 7 花粉解冻 将超低温保藏的花粉冷冻管，置于 4℃条件下解冻 6 h～8 h，再将花粉冷冻管置于自来水水流中冲 洗10 min～15 min，分装到授粉管中待用。 8 花粉回收 当日授粉结束后，将授粉管中剩余的花粉集中到冷冻管中密封，置于 4 ℃条件下保藏可下次使用， 3 d～5 d内使用完。