DB61
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DB611T 409-2007
2007-09-03~1b 2007-09-03~nm
DB61rr 409-2007
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DIN 10476 tfWj$U ~n 9=J.fi~MHll&~ {if!4~1tRtfJlYl~
IDF 97A tfWj#liJ &~Lmf)}9=J{if!4!'fittLf8jl)l~j£
NADP ~tw~)tH:~J1~n~1lt =*~tr~ (trBIW II )
NADPH ~.~5.*~~~&i(~~~.IWU)
±b~, Rt.! nilli M> .. H71+: iIfi:tjFAD ~~I.J)J(?:R ~-1·i'< j ~
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NR
M~1tR + NADPH + H__ ......\niJi~~:tk + NADP + H~O
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5. 1 ~~tiHP;'&:
5. 2 NADP
5.3 FAD
5.4 NR
5.5 Hel
5.6 NaGH
5. 7 KGB
5. 8 :mJ~)J<
DB611T 409-2007
5. 9 .!lEt~1l1.ttlfl K1Fe (CN) n • 3H20
5. 10 i1U§it$ Z"S01 • 7H20
5. 11 1:!!Rf-1tff.ljiij ~ilt*IJ1i
6. 1 ft~*ft (5 mL 1 mL 50-200 j.l L, 10-50 j.l L)
6.2 btEilll: 7't1£ 1. Oem
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6.4 lJT1f;;rz:tK, JL1£ 10 j.l rn 0
6. 5 fJlJ'I),tJl
6. 6 i~H:'\l~\ ~;ft, ft~H~J!j[:,:~y~=*, .JllZ~;W~1;f0:}Y1~j7~
7. 1 ilt1fIJ!ciM
7. 1. 1 ~~!i;IPRf
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7. 1. 2 NADP I1t;-&
m ImL Q;t(~~i"P~~§f(fl O.5mgNADP, tj:mLfll O.Olmg FADo t!P);} NADP yWo
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7. 1. 4 He I: 2 mo I lLo
7.1.5 NaOH: 2moi/Lo
7.1.6 KOH: 2moi/Lo
7. 1. 7 Carrez ;~Rf
7. 1.8 Carrez-I i~~
85mmolIL Carrez- I m~: ~Jtx 3.60g I(4Fe (CN) 6· 3H20, 1.mA 100mL *~.I./<.m~lf.c
7. 1. 8. 1 Carrez- II m~
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7. 2. 2 1tiIi*
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IttUH'tW (20-2S·C), hilA 6 mL Carrez ~ff.m., mNaOH iJ6l pH 8, tt~~w:*birp, mM~*JE
?f.l-J 100mL. ¥M';"J, ][{IM"fDOmin, i1~IE: (;j:ij.~~~iTh)LmLJf:L), J:l:jr),£r~~~Y!~o
7. 3 *1t:j~IJ (t£~ 1ilHT)
7.3. 1 i.i~s~1~~
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~l{V)*7't&m (AI)' nhOA 0.050 mL M~Mil£.ltI"iiH~j-¥4m, 1~¥t~tr&rw.%JiX: (A~~ 40min), i!tltt
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7.3.2 ~~
t[~l:l::Ellil, f1XtXIJIlA 1.000mLNADP I.{t~, O.lOOmLfl~d~~, 1.900mLiI!:~JJ<.¥bttJ 3minJR
i9;.EfX PllYt&111 (AI)' flfbuA 0.050 mL 1i¢jWR.I&~f!ifHrl;l'f4PJ, f}Y1hlttJxrv.Jcnli: (A~~ 40min), i.'1;lt'Z!l},Z
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DB61-T 409-2007 牛奶制品中硝酸盐的测定—酶法 陕西省
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