DB61
1i f~ 1*
DB61/T 413-2007
t
I
2007-09-03~~ 2007-09-03~~
DB611T 413-2007
*tiFft J:f:ll¥J ~t~A~ ~*,H1~HjL
*~ft~J:f:l~A~~~~M®mA~~.~~~.m*acQ
*~ft~~~N: J:f:l~A~~~~~®mA~~.~~~Q
I *~ft~~A: ~*~'~~*'*WW'~~~Q
*tjf,ftJktt~~;fp Q
I
I I i I I I ! i
i ~
! j I
I
!• ,~
J
l;
i
~
I
I
DB61fT 413-2007 t
f
I t
l
1
;:$: tift/fUJI! 7l'if¢f~WlulE11A*4* D-:3jZ *mUfJ~iitl¥r1J~o
*fiftttE m mTiX*4*D-1f:*~~itW!IJlEo
2 tJUiz31 iJ Imjc14
r:yux1LI=* i¥J~~jffiJi*ti~ttEi¥J iJ! )..trn'fi Jj~J~ ;:$:t;j;ttEi¥J,} ~o J'LM:r:E BjtJji¥J iJ! J+1 ~ft~:, ;ltPiQfr§ J'Ji:tHi1
.a.(~~~~~i¥J~~)~.rr~~~mmT*~ttE,~W,.a.M*~ttE~~~~i¥J*~~~
M~"EJ1~ffli3:~X11:i¥J1iifA&;:$:o fLfik~ff BWHfJt}rm~fll:, ;ltiid'#rA&;:$:JffJ::rFf;:$:flFttEo
EN 12138: ;J\.~*UJli~rtcpD-1j'i:~Il1tti¥Jl'if¢W!~1E
. D-MDH D1j'Z*llil1i1i:lll?tI.~
NAD :tj!JfiIi£~~~,* -A~1rllil
NADH f3 -':li!Iw:-~n~ut- '--':'i%tfllil~
U ~mIJ[ ~~lF Jfl-f.fr., if 25·C i¥J fij\ It~14: ftJ= 5} ~ 1 U i¥J ~m~it.$tf ft
PX; 1 ~ mol i¥J~~ 0
A 11& 7tf1tiEi:
C
~r9tR~*JJt
hepes 4-f5:z..~IJJ&~ z:.~jl'iml
I J
4 JliHf
D-1jL ~~ (D-~*~tik) {tD1jL *~1i1i:~£~JW (D-MDH) Hi£i¥Jt~{5r.F, i!liJiNAD~ f{:,7~~lP=mz..~tiko
lltmz..lliliiL0:: liP EE ffiJtf i¥J~{{tft?Hff. PX;jA]Iiil~1i1i:*11 ~.:~ftfWt 0
D-MDH, dec arb J ) D-~*~1i1i: + NAD ... p;jm1~~1i1i: + CO:c + NADH + H"
NADH i¥J ,~, ffi D-1jL*~!Ii i¥J ,~, :!tit11: 0 II
NADHi¥J~id: rn tE334, 340!&365nm4}{!\"· r' i¥J1l'&7tJJ[{Ei:i¥J-'f:t>J~YJ3H~L tI I
5 l"it1fIJ
f 5. 1 hepes
5.2 NAD
5.3 D-MDHI !
5.4 NaClI !5.5 KCl
I
DB61!T 413~2007
5.6 Na2HP04. ·2H2O
5. 7 lim'.
5.8 HCl
5.9 NaOH
5.10 KOH
5. 11 :m:~*
5.12 1Bj:ij"itffl jij&litillj~
6 iii
6.1 nRl#t (5 mL, 1 mL 50-200ILL 10-50IlU
6. 2 ~t~.nn.: :7\:;~£ 1.0em
6. 3 ~91'~*:7\:;Jlit, r:iJS£iJt*, tE 340nm JIJ:AE
6. 4 jJf:ll~~, 11~ 10 11m
6. 5 J§i IL',ll
6. 6 ~IL'i~< ~tf, ~~jtIL n;~*ft~G~;~~, J.&JIii~~
7 ~jmIJ-WiJJ
7. 1 lit1f1J ~ililj
7. 1. 1 hepes ~;,*,j~
1'£90 mL:m:~*r:p:5t!tlIJ:JJO/\0.8 g NaCL 0.037 g KCL 0.0135 g NazHPO,·2H 20, 0.1 g 1i1rfJ~,
0.5 g hepes, 3'EJh~fft¥Jfi, J.j:j NaOH~~ pH 7.05, 100mlo
7.1.2 NAD I1-t:Hi
ImL l1iJ~*mflllf. 52. 5rng f1)}&P:1J NAD T1t:~, -15"C --20·C1:*tfo
7.1.3 D-MDH I1-t:Rl
0.6 mL .ffi:~*?$M 8U Er-Jf;f)}~P;k) D1ji:lti:l~Hl;:Jmtl.!W.I~ 1t~, -15'C --20·C1*#o
7. 1.4 HCl: 2 mollL
7. 1. 5 NaOH: 2mollL
7.1.6 KOH: 2mollL
7.2 litt$ililji
7. 2. 1 1C~§t;~~~;t
J+j KOH llWl~ 25rnl m:H pH fll~ 7~8. :J.mm:l.J~iJj~.1im~. 1lJ:H 1. 000 L 800 mL ~~iJ11ft@:i1I!uo
7. 2. 2 ;~~!ll=*5t*$&
:i1~¥*1!l:!.ffRn, !'iH:", lfXLm~ 25ml. mKOH llWl~ pH 7-8, :JJQM!~*1imff, 1fJtJ L 000 ~
1. 800 mL ;IJt~iJ1lJt@:~W 0
7.2.3 *CO 2 ~f*=*jtf$&
~~:t:ffr~ i¥J CO~~1*, M~:JJQ NaOH l£ KOH. i)aJ','i pH 7-8. Jt~~ff.kg~ 15 ?t~. 1fJH 1. 000
1. 800 mL ¥);~~iJ11Tt@:~!~ 0
7. 3 f&JIJ (t~~ IltHT)
7.3. 1 ii:£S)!1~~
2
DB61-T 413-2007 饮料中D-苹果酸的测定—酶法 陕西省
文档预览
中文文档
6 页
50 下载
1000 浏览
0 评论
309 收藏
3.0分
温馨提示:本文档共6页,可预览 3 页,如浏览全部内容或当前文档出现乱码,可开通会员下载原始文档
本文档由 人生无常 于 2024-10-27 11:45:11上传分享