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EN 1138-1995 7J<JtHt*Uli~rutif'L-:ijL~~*:l\VJl~;E
L-MDH
NAD
NADH
GOT
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mioI L-MDH f :;;;;:......======= ......~ oxaloacetate + NADH + H+ (1) L-Malate + NAD+
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GOT! i (2) Oxaloacetate + L-glutama .::;;;:x:======= ......~ L-aspartate + 2-oxoglutarate
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DB61 rr 414-2007
5. 4 ~It.:e:. Z..*lii.
5.5 L-MDH
5.6 HCl
5.7 NaOH
5.8 KOH
5.9 tii*
5. 1OteiiIitIf)iii8f.lit1lJj*
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6. 1 Uji~ (5 mL 1 mL 50-200~L 10-50~L)
6. 2 ~t1!.no.::)'tt£ LOcm
6. 3 ~9~~:)'t:)'tJllt, iiIs£jtiE:, f£ 340nm ~IJ:iE
6. 4 . mAi.jm, :fLf£ 1 0 ~
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7.1.2 NAD If1:Hl
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7.1.5 HCl: 2 mollL
7.1.6 NaOH: 2molIL
7.1.7 KOH: 2molIL
7. 2 lit~*IJ.
7.2. 1 7[;1!~i~1!~jt
ffl2mol/L KOH i]aJ:lHOmL*rrpH11I~7'"""8, 1JQ:l:li7JC~~, 1fffll. 000~1. 800 mL~~j£Htil~~o
7. 2. 2 ~j.rtl~8f.
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DB61-T 414-2007 饮料中L-苹果酸的测定—酶法 陕西省
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