ISO INTERNATIONAL STANDARD 20150 First edition 2019-01 Footwear and footwear components Quantitative challenge test method to assess antifungal activity Chaussures etcomposants de chaussure-Méthode detest d'eépreuve quantitatif pour évaluer I'activite antifongique Reference number IS0 20150:2019(E) ISo @ IS0 2019 IS0 20150:2019(E) COPYRIGHTPROTECTEDDOCUMENT IS02019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting below or ISo's member body in the country of the requester. ISO copyright office CP 401: Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +4122 749 0111 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 20150:2019(E) Contents Page Foreword 1 Scope .1 2 Normative references .1 3 Terms and definitions 4 Principle .2 5 Safety .2 6 Apparatus. .2 7 Reagents and culture medium .3 7.1 General 3 7.2 Water. 3 7.3 Malt medium. 3 7.3.1 Composition 3 7.3.2 Preparation 3 7.4 Malt extract agar (MEA) medium. .4 7.4.1 Composition 4 7.4.2 Preparation 4 7.5 Physiological saline (sodium chloride solution) 4 7.5.1 Composition 4 7.5.2 Preparation 4 7.6 Wetting agent (nonionic surfactant) 4 7.7 Buffer solution. 4 7.7.1 Bufferstock. X 7.7.2 Preparation of buffer stock 5 7.7.3 Preparation of buffer solution 5 8 Test microorganisms .5 9 Preparation of test inoculums 5 9.1 5 Indicationsforuseofstrains 9.2 Preparation of inoculums of Candida albicans 6 9.3 Preparation of test spore suspension of filamentous micro-fungi 6 10 Preparationoftestspecimens 6 10.1 General. 6 10.2 Testspecimen 10.3 Pre-treatmentoftestspecimen .7 11 Testprocedure .7 .7 11.1 Summary of test methods 11.2 Dynamic challenge tests 1 .7 11.2.1 Inoculation 11.2.2 Neutralization and elution after inoculation (time o h) 8 11.2.3 Incubation. 8 11.2.4 Neutralization and elution after incubation (time 24 h) 8 11.2.5 Determination of thenumberofviablemicro-fungi 8 11.3 Static challenge test. 8 11.3.1 Inoculation 8 11.3.2 Neutralization and elution after inoculation (time o h) .9 11.3.3 Incubation. 9 11.3.4 Neutralization and elution after incubation (time 24 h) .9 11.3.5 Determination of thenumberofviablemicro-fungi 9 12 Expression of results 9 12.1 Calculation ofthenumberofviablemicro-fungi 9 12.2 Judgementoftesteffectiveness. .9 @ IS0 2019 - All rights reserved iii